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Anti-NR2F2 Antibody

Catalog Number: orb256726

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb256726
CategoryAntibodies
DescriptionRabbit polyclonal antibody to NR2F2
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Porcine, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NR2F2. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetNR2F2
Entrez113984, 7026, 11819
UniProt IDP24468, O09018, P43135
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti ARP1 antibody, anti TFCOUP2 antibody, anti CO
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-NR2F2 Antibody

Western blot analysis of NR2F2 expression in HepG2 (A), HeLa (B) whole cell lysates. (Predicted band size: 45 kD; Observed band size: 45 kD)

Anti-NR2F2 Antibody

Immunohistochemical analysis of NR2F2 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-NR2F2 Antibody

Immunofluorescent analysis of NR2F2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

  • ARP-1 rabbit pAb [orb764581]

    ELISA,  IF,  IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • NR2F2 Antibody [orb670546]

    ELISA,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg
  • NR2F2 peptide [orb257016]

    Blocking

    > 85%

    Synthetic

    5 mg, 1 mg