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Anti-Notch1 APC

Catalog Number: orb154447

DispatchUsually dispatched within 5-10 working days weeks.
$ 330.00
Catalog Numberorb154447
CategoryAntibodies
DescriptionMouse monoclonal antibody conjugated to APC which recognizes Notch 1 that belongs to the NOTCH family transmembrane receptors that play important roles in development and the determination of cell fate. It expressed in fetal tissues most abundant in spleen, brain stem and lung. Notch 1 has functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination.
ClonalityMonoclonal
Clone NumbermN1A
Tested applicationsFC
ReactivityHuman, Mouse
IsotypeMouse IgG1 kappa
ImmunogenGST fusion protein containing cdc10-NCR region of mouse Notch1
Concentration0.1 mg/ml
PurityPurified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
ConjugationAPC
TargetNotch1
Entrez4851
UniProt IDP46531
StorageStore at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/PreservativesStabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Alternative namesAnti-AOS5 antibody, anti-TAN1 antibody, anti-hN1 a
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NoteFor research use only
Application notesFlow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Expiration Date12 months from date of receipt.
Anti-Notch1 APC

Separation of MOLT-4 cell line (red) from Leukocytes(blue) in flow cytometry analysis (intracellular staining) stained using anti-human Notch1 (mN1A) APC antibody (concentration in sample 3 µg/ml).

Anti-Notch1 APC

Flow cytometry multicolor surface staining pattern of human PHA stimulated CD3 positive lymphocytes using anti-human CD25 (MEM-181) PE antibody (20 µl reagent / 100 µl of sample) and intracellular staining of human lymphocytes using anti-Notch1 (mN1A) APC antibody (concentration in sample 3 µg/ml).

Anti-Notch1 APC

Separation of CD3 positive CD25 positive cells stained using anti-Notch1 (mN1A) APC antibody (concentration in sample 3 µg/ml, red-filled) from CD3 positive CD25 positive cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 3 µg/ml, same as Notch1 APC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of PHA stimulated human peripheral whole blood.

Anti-Notch1 APC

Flow cytometry intracellular staining patterns of PHA stimulated human peripheral whole blood stained using anti-Notch1 (mN1A) PE antibody (concentration in sample 3 µg/ml, left) or mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 3 µg/ml, same as Notch1 PE concentration, right).