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Anti-NOTCH1 Antibody

Catalog Number: orb256723

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb256723
CategoryAntibodies
DescriptionRabbit polyclonal antibody to NOTCH1, which is also known as AOVD. NOTCH1 is frequently found in the intracellular domain of the nucleus and is expressed in the basal keratinocytes and mesencephalic ventricular zone of the neural tube. It is overexpressed in the cerebrospinal fluid. This protein extensively functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human NOTCH1. The exact sequence is proprietary.
Dilution rangeWB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetNOTCH1
Entrez25496, 18128, 4851
UniProt IDQ07008, Q01705, P46531
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti-TAN1 antibody, anti-Neurogenic locus notch ho
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-NOTCH1 Antibody

Western blot analysis of NOTCH1 expression in Myla2059 (A) whole cell lysates. (Predicted band size: 272 kD; Observed band size: 120 kD)

Anti-NOTCH1 Antibody

Immunohistochemical analysis of NOTCH1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-NOTCH1 Antibody

Immunofluorescent analysis of NOTCH1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • Anti-Notch1 APC [orb154447]

    FC

    Human, Mouse

    Monoclonal

    APC

    0.1 mg
  • Anti-Notch1 PE [orb154505]

    FC

    Human, Mouse

    Monoclonal

    PE

    0.1 mg
  • Goat anti-NOTCH1 (aa171-182) Antibody [orb421981]

    ELISA,  IF,  IHC

    Bovine, Canine, Human, Mouse, Rat

    Goat

    Polyclonal

    Unconjugated

    100 μg
  • Anti-NOTCH1 Antibody [orb1422740]

    IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • Cleaved-NOTCH1 (V1754) Antibody [orb675227]

    ELISA,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 50 μg