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Anti-NOTCH1 Antibody

Catalog Number: orb1422740

DispatchUsually dispatched within 5-10 working days
$ 600.00
Catalog Numberorb1422740
CategoryAntibodies
DescriptionRabbit polyclonal antibody to NOTCH1.
Species/HostRabbit
ClonalityPolyclonal
Clone NumberNOTCH1
Tested applicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
Dilution rangeWB: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
ConjugationUnconjugated
UniProt IDP46531
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesTAN1; Neurogenic locus notch homolog protein 1; No
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-NOTCH1 Antibody

Immunofluorescent analysis of NOTCH1 staining in HeLa Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Anti-NOTCH1 Antibody

Immunohistochemical analysis of NOTCH1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-NOTCH1 Antibody

Western blot analysis of NOTCH1 expression in Myla2059 (A) whole cell lysates.

  • Anti-Notch1 APC [orb154447]

    FC

    Human, Mouse

    Monoclonal

    APC

    0.1 mg
  • Anti-Notch1 PE [orb154505]

    FC

    Human, Mouse

    Monoclonal

    PE

    0.1 mg
  • Goat anti-NOTCH1 (aa171-182) Antibody [orb421981]

    ELISA,  IF,  IHC

    Bovine, Canine, Human, Mouse, Rat

    Goat

    Polyclonal

    Unconjugated

    100 μg
  • Anti-NOTCH1 Antibody [orb256723]

    IF,  IH,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 30 μl
  • Cleaved-NOTCH1 (V1754) Antibody [orb675227]

    ELISA,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 50 μg