Anti-nNOS Antibody

• Catalog Number: orb214323
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NOS1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human nNOS. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: NOS1
• Entrez: 24598, 18125, 4842
• UniProt ID: P29476, P29475, Q9Z0J4
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Nitric oxide synthase, brain antibody, anti N
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of nNOS expression in HeLa (A), Raw264.7 (B), mouse brain (C), PC12 (D), rat brain (E) whole cell lysates. (Predicted band size: 160 kD; Observed band size: 160 kD)

Immunohistochemical analysis of nNOS staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.