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Catalog Number | orb1743849 |
---|---|
Category | Antibodies |
Description | Anti-NHP2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, WB |
Reactivity | Human |
Immunogen | E.coli-derived human NHP2 recombinant protein (Position: E21-Q136). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 20 kDa |
UniProt ID | Q9NX24 |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Note | For research use only |
Application notes | Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of SH-SY5Y cells using anti-NHP2 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of NHP2 using anti-NHP2 antibody.Lane 1:human SH-SY5Y cell;2:human Caco-2 cell;3:human HeLa cell.
IF analysis of NHP2 using anti-NHP2 antibody and anti-Beta Tubulin antibody.NHP2 was detected in immunocytochemical section of A549 cell.
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