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Catalog Number | orb1148194 |
---|---|
Category | Antibodies |
Description | Anti-NFkB-p65 (Phospho-Thr435) Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Peptide sequence that includes phosphorylation site of threonine 435 (E-G-T(p)-L-S) derived from human NFΰB-p65 and conjugated to KLH. Accession no.: Q04206, NP_001138610.1 Host Species: Rabbit Specificity: This antibody detects endogenous human, mouse, and rat NFΰB-p65 only when phosphorylated at threonine 435. |
Concentration | 1.0 mg/mL |
Dilution range | Western blotting: use at dilution of 1:500-1:1,000. A band of ~65kDa is detected. Immunohistochemistry: use at dilution of 1:50-1:100. Immunofluorescence: use at dilution of 1:100-1:200. These are recommended working dilutions. Enduser should determine optimal dilutions for their applications. |
Form/Appearance | Liquid |
Purity | Purified |
Conjugation | Unconjugated |
Target | NFkB-p65 (Phospho-Thr435) |
Storage | This antibody is stable for at least one (1) year at -20C. Can be stored at 4C for short-term use. |
Buffer/Preservatives | 0.02% Sodium Azide. PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
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