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Anti-MYLK3 Antibody

Catalog Number: orb1993152

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb1993152
CategoryAntibodies
DescriptionAnti-MYLK3 Antibody. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, FC, WB
ReactivityHuman
ImmunogenE.coli-derived human MYLK3 recombinant protein (Position: E34-K812). Human MYLK3 shares 68.8% and 66.8% amino acid (aa) sequence identity with mouse and rat MYLK3, respectively.
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW100 kDa
UniProt IDQ32MK0
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NoteFor research use only
Application notesWestern blot, 0.25-0.5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
Expiration Date12 months from date of receipt.
Anti-MYLK3 Antibody

Flow Cytometry analysis of HEL cells using anti-MYLK3 antibody. Overlay histogram showing HEL cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYLK3 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Anti-MYLK3 Antibody

Western blot analysis of MYLK3 using anti-MYLK3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYLK3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MYLK3 at approximately 100 kDa. The expected band size for MYLK3 is at 88 kDa.

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