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Catalog Number | orb44797 |
---|---|
Category | Antibodies |
Description | Rat monoclonal antibody against CD8 conjugated to FITC |
Clonality | Monoclonal |
Clone Number | 53-6.7 |
Tested applications | FC |
Reactivity | Mouse |
Isotype | Rat IgG2a kappa |
Immunogen | Murine spleen cells |
Antibody Type | Primary Antibody |
Concentration | 0.5 mg/ml |
Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | FITC |
Target | CD8a |
Entrez | 12525 |
UniProt ID | P01731 |
RRID | AB_10999622 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | anti CD 8 antibody (FITC), anti CD 8A antibody (FI Read more... |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 1-2 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Separation of murine CD8a positive splenocytes (red-filled) from CD8a negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD8a (53-6.7) FITC antibody (concentration in sample 1 μg/ml).
Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD8a (53-6.7) FITC antibody (concentration in sample 1 μg/ml).