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Catalog Number | orb623585 |
---|---|
Category | Antibodies |
Description | Rat monoclonal antibody against CD16 conjugated to PE |
Clonality | Monoclonal |
Clone Number | 93 |
Tested applications | FC |
Reactivity | Mouse |
Isotype | Rat IgG2a lambda |
Immunogen | Murine pre-B cells |
Antibody Type | Primary Antibody |
Concentration | 0.5 mg/ml |
Dilution range | FACS: 2 μg/ml |
Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | PE |
Target | CD16/CD32 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-CD16 antibody, anti-CD32 antibody, anti-Fcgam Read more... |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 2 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Separation of murine CD16/32 positive cells (red-filled) from murine CD16/32 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD16/32 (93) PE antibody (concentration in sample 5 µg/ml).
Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD16/32 (93) PE antibody (concentration in sample 5 µg/ml).