You have no items in your shopping cart.
Cart summary
Item 1 of 1
Anti-Mouse MIP-3 alpha (RAT) Biotin Conjugated Antibody
Catalog Number: orb345245
| Catalog Number | orb345245 |
|---|---|
| Category | Antibodies |
| Description | MIP-3 alpha (RAT) antibody (Biotin) |
| Species/Host | Rat |
| Clonality | Monoclonal |
| Clone Number | 4N5F7 |
| Tested applications | ELISA, IP, NeA, WB |
| Reactivity | Mouse |
| Isotype | IgG |
| Immunogen | Anti-MIP-3α (RAT) Monoclonal Antibody was produced in rat by repeated immunizations with mature full length recombinant mouse MIP-3α produced in E.coli followed by hybridoma development. |
| Concentration | 1.0 mg/mL |
| Dilution range | WB: 0.5µg/mL |
| Form/Appearance | Lyophilized |
| Purity | This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse MIP-3α protein. A BLAST analysis was used to suggest cross-reactivity with MIP-3α from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with MIP-3α from other sources has not been determined. |
| Conjugation | Biotin |
| UniProt ID | O89093 |
| NCBI | NP_001153210.1 |
| Storage | Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | rat anti-MIP-3α biotin conjugated antibody, CCL20, Read more... |
| Note | For research use only |
| Application notes | This purified antibody has been tested for use in ELISA, IP, western blot, and Neutralization. Specific conditions for reactivity should be optimized by the end user. |
| Expiration Date | 12 months from date of receipt. |
Western Blot of Mouse MIP 3α (RAT) antibody Lane 1: mouse recombinant MIP-3a Lane 2: human recombinant MIP-3a Primary antibody: MIP 3α antibody at 0.1 ug/ml for overnight at 4°C Secondary antibody: IRDye800™ goat anti-rat at 1:10000 for 45 min at RT Block: 5% BLOTTO overnight at 4°C
