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Catalog Number | orb545718 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to MICA/MICB |
Clonality | Monoclonal |
Clone Number | 6D4 |
Tested applications | FC, IHC, IP |
Reactivity | Human |
Isotype | Mouse IgG2a |
Immunogen | Transfected C1R cells expressing MICA |
Antibody Type | Primary Antibody |
Concentration | 1 mg/ml |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | MICA/MICB |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 1-4 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Separation of human HeLa cells (red-filled) from leukocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood spiked with HeLa cells stained using anti-human MICA/MICB (6D4) purified antibody (concentration in sample 0.3 μg/ml, GAM APC).
Flow cytometry surface staining pattern of human peripheral whole blood spiked with HeLa cells stained using anti-human MICA/MICB (6D4) purified antibody (concentration in sample 0.3 μg/ml, GAM APC).