Anti-MICA antibody(7F12), IgG1 Chimeric mAb

• Catalog Number: orb2322346
• Category: Antibodies
• Description: Anti-MICA antibody(7F12), IgG1 Chimeric mAb
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: 7F12
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Rabbit/Human Fc chimeric IgG1
• Antibody Type: Recombinant Antibody
• Dilution range: WB 1:1000; Flow Cyt 1:100
• Conjugation: Unconjugated
• Target: MICA
• UniProt ID: Q29983
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MIC-A; PERB11.1
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

Anti-MICA antibody (SKU# orb2322346) at 1/1000 dilution.Lane: HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.band size: 43 kDa

Flow cytometry analysis of antigen binding of anti-human MICA mAb (orb2322346). (A) orb2322346 does bind to PC3 cells that weakly express MICA. (B) A clear peak shift of orb2322346 was seen compared to the control when incubated with MICA-expressing Hela cells, indicating strong binding of orb2322346 to MICA. Antibodies were incubated at 10 μg/mL.