Anti-MGMT Antibody

• Catalog Number: orb1473977
• Category: Antibodies
• Description: Mouse monoclonal antibody to MGMT
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human MGMT. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50)
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MGMT
• Entrez: 4255
• UniProt ID: P16455
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Methylated-DNA--protein-cysteine methyltransferase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MGMT expression in Jurkat (A), MCF7 (B), MOLT4 (C), Raji (D) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 22 kD)

Flow cytometric analysis of Jurkat cells using Anti-MGMT Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.