Anti-MAD2L2 Antibody

• Catalog Number: orb1743766
• Category: Antibodies
• Description: Anti-MAD2L2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human
• Immunogen: E.coli-derived human MAD2L2 recombinant protein (Position: M1-K209).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 24 kDa
• UniProt ID: Q9UI95
• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Note: For research use only
• Application notes: Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HeLa cells using anti-MAD2L2 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of MAD2L2 using anti-MAD2L2 antibody.Lane 1:human A375 cell;2:human 293T cell;3:human HeLa cell;4:human K562 cell;5:human Jurkat cell;6:human THP-1 cell;7:human SH-SY5Y Cell lysats.

IF analysis of MAD2L2 using anti-MAD2L2 antibody. MAD2L2 was detected in an immunocytochemical section of U2OS cells.

IHC analysis of MAD2L2 using anti-MAD2L2 antibody. MAD2L2 was detected in a paraffin-embedded section of human liver cancer tissue.