Anti-LCK (pY393) Antibody

• Catalog Number: orb1423486
• Category: Antibodies
• Description: Rabbit polyclonal antibody to LCK (pY393).
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: LCK
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Porcine, Rat, Virus
• Dilution range: WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), E: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
• Conjugation: Unconjugated
• UniProt ID: P06239
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Tyrosine-protein kinase Lck; Leukocyte C-terminal
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of LCK (pY393) expression in Myla2059 (A), HuT78 (B) whole cell lysates.

Direct ELISA antibody dose-response curve using Anti-LCK (pY393) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Immunofluorescent analysis of LCK (pY393) staining in MCF7 Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).