Anti-KEAP1 Antibody

• Catalog Number: orb1474440
• Category: Antibodies
• Description: Rabbit monoclonal antibody to KEAP1
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant protein of human Keap1
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100)
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Conjugation: Unconjugated
• Target: KEAP1
• Entrez: 9817, 50868
• UniProt ID: Q14145, Q9Z2X8
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Alternative names: INRF2; KIAA0132; KLHL19; Kelch-like ECH-associated
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of KEAP1 expression in Raw264.7 (A), Hela (B), A549 (C), C2C12 (D) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 60-64 kD)

Immunohistochemical analysis of KEAP1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.22). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.