Anti-IL-6 receptor [rhPM-1 (Tocilizumab)]

• Catalog Number: orb396139
• Category: Antibodies
• Description: Mouse monoclonal antibody to IL-6 receptor.
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: rhPM-1 (Tocilizumab)
• Tested applications: ELISA
• Reactivity: Human
• Isotype: IgG1
• Immunogen: The original antibody was generated by CDR grafting of mouse PM-1 antibody onto human REI and NEW framework regions to create rhPM-1.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: IL-6 receptor
• UniProt ID: P08887
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: IL6R; IL-6R; CD126; MRA; atlizumab; TCZ; Interleuk
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Sato, K. et al. Humanization of a mouse anti-human interleukin-6 receptor antibody comparing two methods for selecting human framework regions Mol Immunol, 31, 371-381 (1994)

PubMed: 8152440

Immunofluorescence staining of fixed U937 cells with anti-IL-6 receptorantibody rhPM-1 (Tocilizumab). Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of rhPM-1 (Tocilizumab) (orb396144) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb396144, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.