Anti-IL-4 [SB240683 (Pascolizumab, humanized 3B9)]

• Catalog Number: orb2645820
• Category: Antibodies
• Description: Anti-IL-4 [SB240683 (Pascolizumab, humanized 3B9)]
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: SB240683 (Pascolizumab, humanized 3B9)
• Tested applications: Blocking, ELISA, FA
• Reactivity: Human, Monkey
• Isotype: IgG
• Immunogen: The original mouse antibody was generated by immunizing F1 hybrids of Balb/c and C57BL/6 mice with recombinant human IL-4 in Freunds complete adjuvant. The humanized version was generated by grafting CDRs of murine parent antibody onto human IgG1 kappa heavy- and light-chain frameworks.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: IL-4
• UniProt ID: P05112
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: IL4; BSF-1; Interleukin-4; B-cell stimulatory fact
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using anti-IL-4 antibody SB240683 (Pascolizumab, humanized 3B9). Human blood leukocytes rested for 4 hours at 37°C, then fixed with 2% PFA and permeabilized with 0.5% Triton, were stained with the anti-unknown specificity antibody (orb256458; isotype control, right) or the rabbit IgG version of SB240683 (orb2645820, left) at a dilution of 1:100 overnight at 4°C. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Daudi cells with anti-IL-4 antibody SB240683 (Pascolizumab, humanized 3B9). Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of SB240683 (Pascolizumab, humanized 3B9) (orb2645820) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cellular junction staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645820, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.