Anti-IL-2R [Daclizumab]

• Catalog Number: orb1152523
• Category: Antibodies
• Description: Mouse monoclonal antibody to IL-2R
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: Daclizumab
• Tested applications: Blocking, FC
• Reactivity: Human, Primate
• Isotype: Human IgG1
• Immunogen: Cultured T-cells derived from a patient with Mycosis fungoides.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: IL-2R
• UniProt ID: P01589
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD25; Interleukin-2 receptor subunit alpha; IL-2 r
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using anti-IL-2R antibody Daclizumab Cynomolgus monkey lymphocytes were stained with an isotype control (orb256390, red) or the rabbit-chimeric version of Daclizumab (orb348909, blue) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FlowJo single-cell flow cytometer.

Flow-cytometry using anti-IL-2R antibody Daclizumab Rhesus monkey lymphocytes were stained with an isotype control (orb256390, red) or the rabbit-chimeric version of Daclizumab (orb348909, blue) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FlowJo single-cell flow cytometer.

Flow-cytometry using the anti-CD25 (IL2R) research biosimilar antibody Daclizumab Human lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Daclizumab (orb348909, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Immunohistochemical staining of rat spleen using anti-IL2R antibody. Formalin fixed rat spleen slices were were stained with orb348866 at 5 µg/ml.

Western Blot using anti-IL2R antibody. Mouse lipocyte (lane 1), mouse spleen (lane 2), mouse thymus (lane 3), mouse lymph node (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with orb348866 at 1.5 µg/ml before being detected by a secondary antibody. The expected band size (31 kDa) is seen in the human-derived sample, as well as further bands that are specific to human.