Anti-IgM [M15/8]

• Catalog Number: orb758769
• Category: Antibodies
• Description: Rabbit monoclonal antibody to IgM
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: M15/8
• Tested applications: Blocking, ELISA, FC, IHC-Fr, IP
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: BALB/c mice immunized with human IgM.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: IgM
• UniProt ID: P01871
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: mu-chain
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA of anti-IgM antibody on IgM Fc region peptide and a murine IgM antibody. Binding curves of the rabbit chimeric version of the anti-IgM antibody M15/8 (orb758769; red line) and isotype control (anti-Fluorescein atnibody; orb256390; blue line) to an ELISA plate coated with either IgM Fc region peptide at a concentration of 5 µg/ml (A) or murine IgM antibody (B; Anti-CD41 antibody) again at 5 µg/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using orb758769. For signal detection, a 1:4000 dilution of HRP-labelled anti-rabbit IgG1 antibody was used. Both IgM Fc region peptide (monomeric ligand) and murine IgM antibody (multimeric ligand) were recognised by orb758769.

Flow-cytometry using the anti-IgM M15/8 Daudi cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M15/8 (orb758769, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.

Western Blot using anti-IgM antibody M15/8 Human spleen lysate (35 µg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of M15/8 (orb758769) at 0.3 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for IgM is 180/900kDa for monomeric/pentameric IgM respectively.