Anti-IGF1 Receptor (Phospho-Y1161) Antibody

• Catalog Number: orb224171
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IGF1 Receptor (Phospho-Y1161)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, IP, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1161 of human IGF1 Receptor protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:200, IF/ICC: 1:100-1:500, IP: 1:10-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: IGF1R
• Entrez: 16337, 25718, 3643, 16001, 3480
• UniProt ID: P15208, P06213, Q60751, P15127, P08069, P24062
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti IGF1R antibody, anti Insulin-like growth fact
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IGF1 Receptor (Phospho-Y1161) expression in zebrafish (A) whole cell lysates. (Predicted band size: 154; 156 kD; Observed band size: 200 kD)

Immunohistochemical analysis of IGF1 Receptor (Phospho-Y1161) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of IGF1 Receptor (Phospho-Y1161) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).