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Anti-Hu CD77 PE
Catalog Number: orb1088485
| Catalog Number | orb1088485 |
|---|---|
| Category | Antibodies |
| Description | CD77 (globotriaosylceramide Gb3), also known as the Pk blood group antigen, BLA (Burkitt´s lymphoma associated antigen), or CTH (ceramide trihexoside) is a neutral glycosphingolipid composed of three carbohydrate molecules linked to a lipid moiety in the cell membrane (Gal-alpha1-4Gal-beta1-4Glc-beta1-Cer). It is expressed on germinal center B cells, Burkitt´s lymphoma cells, it can be induced on extrafolicular B cells and it is also found on endothelia and epithelia. CD77 may be involved in elimination of germinal center B cells that fail to produce high affinity antibodies, and serves also as receptor for shiga toxin and verotoxin. |
| Clonality | Monoclonal |
| Clone Number | 38.13 |
| Tested applications | FC |
| Reactivity | Human |
| Isotype | Lewis Rat IgM |
| Immunogen | Daudi cell line (Burkitt´s lymphoma) |
| Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Conjugation | PE |
| Target | CD77 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Buffer/Preservatives | Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Alternative names | Anti-globotriaosylceramide Gb3 antibody, anti-Gal- Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Expiration Date | 12 months from date of receipt. |
Separation of RAJI cells stained using anti-human CD77 (38.13) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from RAJI cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 5 μg/ml, same as CD77 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of RAJI cell suspension.
