Anti-Hu CD54 Purified

• Catalog Number: orb44030
• Category: Antibodies
• Description: Mouse Monoclonal to CD54.
• Clonality: Monoclonal
• Clone Number: MEM-111
• Tested applications: ELISA, FC, ICC, IHC-P, WB
• Reactivity: Bovine, Human, Rat
• Isotype: Mouse IgG2a
• Immunogen: Raji human Burkitt's lymphoma cell line
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD54
• Entrez: 3383
• UniProt ID: P05362
• RRID: AB_10996504
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD54 antibody, anti-ICAM-1 antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 2 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: thymus, RE cells. Western blotting: Positive control: RAJI cell line K562 leukemia cell line, JY cell line, aa ctivated T-lymphocytes, non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of CD54 expression in TNF-alpha activated (A) and nonactivated (B) HUVEC cells by antibody MEM-111. Lower bands represent tubulin as a loading control.

Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD14 (MEM-15) PE antibody (20 µl reagent / 100 µl of peripheral whole blood) and anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).

Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).

Western blotting analysis of human CD54 using mouse monoclonal antibody MEM-111 on lysates of TF-1 and Raji cells, as well as of JURKAT cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD54 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for CD54 at approximately 88 kDa.