Anti-Hu CD54 Purified Azide Free

• Catalog Number: orb43579
• Category: Antibodies
• Description: Mouse Monoclonal to CD54.
• Clonality: Monoclonal
• Clone Number: 1H4
• Tested applications: FC, ICC, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Raji cells and spleen cells fused with NS1 cells
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (frozen sections): Recommended dilution: 5-10 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; prolonged fixation in buffered formalin can destroy the epitope. High temperature antigen unmasking technique is required. Flow cytometry: Recommended dilution: 1-4 μg/ml. Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD54
• Entrez: 3383
• UniProt ID: P05362
• RRID: AB_10995858
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: Anti-CD54 antibody, anti-ICAM-1 antibody
• Note: For research use only
• Application notes: Immunohistochemistry (frozen sections): Recommended dilution: 5-10 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; prolonged fixation in buffered formalin can destroy the epitope. High temperature antigen unmasking technique is required. Flow cytometry: Recommended dilution: 1-4 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD54 (1H4) purified antibody (concentration in sample 3 μg/ml, GAM FITC).

Western blotting analysis of human CD54 using mouse monoclonal antibody 1H4 on lysates of TF-1 and Raji cells, as well as of JURKAT cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD54 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for CD54 at approximately 88 kDa.

Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD54 (1H4) purified antibody (concentration in sample 3 μg/ml, GAM FITC).