You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb154477 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody conjugated to FITC which recognizes CD314 that is also known as BAT221, NKG2D a cell surface receptor of the type II transmembrane receptor NKG2 family. CD314 is expressed on NK cells and some T cell subsets, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion. |
Clonality | Monoclonal |
Clone Number | 1D11 |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgG1 kappa |
Immunogen | NKL cell line |
Antibody Type | Primary Antibody |
Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | FITC |
Target | CD314 |
Entrez | 22914 |
UniProt ID | P26718 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-NKG2D antibody, anti-KLRK1 antibody, anti-KLR Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD314 (1D11) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood) and anti-human CD56 (LT56) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).
Separation of human CD314 positive CD56 positive NK cells (red-filled) from CD314 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD314 (1D11) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD314 (1D11) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood).