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Anti-Hu CD314 FITC

Catalog Number: orb154477

DispatchUsually dispatched within 5-10 working days weeks.
$ 330.00
Catalog Numberorb154477
CategoryAntibodies
DescriptionMouse monoclonal antibody conjugated to FITC which recognizes CD314 that is also known as BAT221, NKG2D a cell surface receptor of the type II transmembrane receptor NKG2 family. CD314 is expressed on NK cells and some T cell subsets, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion.
ClonalityMonoclonal
Clone Number1D11
Tested applicationsFC
ReactivityHuman
IsotypeMouse IgG1 kappa
ImmunogenNKL cell line
Antibody TypePrimary Antibody
PurityPurified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
ConjugationFITC
TargetCD314
Entrez22914
UniProt IDP26718
StorageStore at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/PreservativesStabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Alternative namesAnti-NKG2D antibody, anti-KLRK1 antibody, anti-KLR
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NoteFor research use only
Application notesFlow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Expiration Date12 months from date of receipt.
Anti-Hu CD314 FITC

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD314 (1D11) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood) and anti-human CD56 (LT56) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Anti-Hu CD314 FITC

Separation of human CD314 positive CD56 positive NK cells (red-filled) from CD314 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD314 (1D11) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood).

Anti-Hu CD314 FITC

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD314 (1D11) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood).