Anti-Hu CD273 Purified

• Catalog Number: orb402782
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD273
• Clonality: Monoclonal
• Clone Number: 24F.10C12
• Tested applications: FC, IHC
• Reactivity: Human
• Isotype: Mouse IgG2a kappa
• Immunogen: human CD273 cDNA
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD273
• Entrez: 80380
• UniProt ID: Q9BQ51
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-PDCD1LG2 antibody, anti-B7DC antibody, anti-B
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 µg/ml
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) monocytes using anti-human HLA-DR (L243) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD273 (24F.10C12) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) monocytes using anti-human CD11c (BU15) Pacific Blue™ antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD273 (24F.10C12) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.

Separation of human CD11c positive HLA-DR positive cells (red-filled) from CD11c negative HLA-DR negative cells (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) monocytes stained using anti-human CD273 (24F.10C12) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) monocytes stained using anti-human CD273 (24F.10C12) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.