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Item 1 of 5
Anti-Hu CD142 Purified
Catalog Number: orb402784
| Catalog Number | orb402784 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to Tissue factor |
| Clonality | Monoclonal |
| Clone Number | HTF-1 |
| Tested applications | FC, ICC, IHC-P, WB |
| Reactivity | Human |
| Isotype | Mouse IgG1 kappa |
| Immunogen | Human brain tissue factor (CD142) |
| Concentration | 1 mg/ml |
| Dilution range | Flow cytometry: Recommended dilution: 3-12 µg/ml. Western blotting: Recommended dilution: 1-2 μg/ml. |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | CD142 |
| Entrez | 2152 |
| UniProt ID | P13726 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-F3 antibody, anti-tissue factor antibody, ant Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: Recommended dilution: 3-12 µg/ml.Western blotting: Recommended dilution: 1-2 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Flow cytometry multicolor surface staining pattern of human PHA stimulated peripheral blood mononuclear cells using anti-human CD142 (HTF-1) purified antibody (concentration in sample 4 μg/ml, GAM APC) and SYTO 16.
Separation of human CD142 positive lymphocytes (red-filled) from CD142 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD142 (HTF-1) purified antibody (concentration in sample 4 μg/ml, GAM APC).
Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD142 (HTF-1) purified antibody (concentration in sample 4 μg/ml, GAM APC).
Immunohistochemistry staining of human cortex (paraffin-embedded sections) with anti-CD142 (HTF-1), 10 μg/ml.
Western blotting analysis of human CD142 using mouse monoclonal antibody HTF-1 on lysates of A431 cell line and MCF7 and THP1 cell lines (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD142 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD142 was detected around 50 kDa.
