Anti-Hu CD14 Purified

• Catalog Number: orb44142
• Category: Antibodies
• Description: Mouse Monoclonal to CD14.
• Clonality: Monoclonal
• Clone Number: MEM-18
• Tested applications: ELISA, FC, IP, WB
• Reactivity: Human, Primate
• Isotype: Mouse IgG1
• Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD14
• Entrez: 929
• UniProt ID: P08571
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD14 antibody, anti-LPS-receptor antibody
• Note: For research use only
• Application notes: ELISA: The antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody B-A8 (cat. no. 11-304-C100). Flow cytometry: recommended dilution: 4 μg/ml. Western blotting: Non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis (non-reducing conditions) of over-expressed human CD14 using anti-CD14 (MEM-18). Lane 1: whole cell lysate HEK 293 transfected with empty vector; Lane 2: tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA; Lane 3: whole cell lysate of HEK 293 transfected with human CD14 cDNA.

Separation of human monocytes (red-filled) from CD14 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD14 (MEM-18) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD14 (MEM-18) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).