Anti-Hu CD14 Purified

• Catalog Number: orb43674
• Category: Antibodies
• Description: Mouse Monoclonal to CD14.
• Clonality: Monoclonal
• Clone Number: B-A8
• Tested applications: ELISA, FC, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Human monocytes
• Concentration: 1 mg/ml
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: CD14
• Entrez: 929
• UniProt ID: P08571
• RRID: AB_10993408
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD14 antibody, anti-LPS-receptor antibody
• Note: For research use only
• Application notes: ELISA: The antibody B-A8 has been tested as the capture antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody MEM-18 (cat. no. 1B-212-C100). Flow cytometry: recommended dilution: 1 μg/ml; Immunohistochemistry (paraffin sections): recommended dilution: 2-10 μg/ml, positive tissue: PML brain sections; positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels. Heat retrieval of antigen is recommeded.
• Expiration Date: 12 months from date of receipt.

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD14 (B-A8) purified antibody (concentration in sample 1.7 μg/ml) GAM APC.