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Item 1 of 4
Anti-Hu CD138 APC
Catalog Number: orb623578
| Catalog Number | orb623578 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against Syndecan 1 conjugated to APC |
| Clonality | Monoclonal |
| Clone Number | MI15 |
| Tested applications | FC |
| Reactivity | Human, Primate |
| Isotype | Mouse IgG1 kappa |
| Immunogen | A mixture of U266 and XG-1 human myeloma cell lines |
| Antibody Type | Primary Antibody |
| Purity | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Conjugation | APC |
| Target | CD138 |
| Entrez | 6382 |
| UniProt ID | P18827 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-syndecan 1 antibody, anti-SYND1 antibody, ant Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Expiration Date | 12 months from date of receipt. |
Staining pattern of Anti-human CD38 FITC antibody (clone HIT2) in dot-plot fluorescence visualization (B cell gate) Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Mouse monoclonal anti-human CD38 FITC antibody (clone HIT2) in concentration 0.5 µg/ml in stained stained blood sample (2 x 10^6 cells) and Mouse monoclonal anti-human CD27 Pacific Blue™ antibody (clone LT27) was used in concentration 2 µg/ml, respectively.
Staining pattern of Anti-human CD38 FITC antibody (clone HIT2) in dot-plot fluorescence visualization (B cell gate) Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Mouse monoclonal anti-human CD38 FITC antibody (clone HIT2) in concentration 0.5 µg/ml in stained stained blood sample (2 x 10^6 cells) and Mouse monoclonal anti-human CD138 APC antibody (clone MI15) in concentration 2 µg/ml, respectively.
Reactivity of Anti-human CD38 FITC antibody (clone HIT2) on human peripheral leukocytes Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Suspension of blood leukocytes (2 x 10^6 cells) was added to the mixture of CD38 FITC antibody (clone HIT2, 0.5 µg/ml in stained blood sample), backbone antibody conjugates and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Stained sample was fixed with 2 ml of 10× diluted EXCELLYSE Easy solution for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.
Anti-human CD38 FITC antibody (clone HIT2) works in flow cytometry application. Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Mouse monoclonal anti-human CD38 FITC antibody (clone HIT2) was used in concentration 0.5 µg/ml in stained blood sample (2 x 10^6 cells).
