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Anti-Hu CD133 APC
Catalog Number: orb545655
| Catalog Number | orb545655 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against CD133 conjugated to APC |
| Clonality | Monoclonal |
| Clone Number | W6B3C1 |
| Tested applications | FC |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Immunogen | WERI-RB-1 retinoblastoma cell line |
| Antibody Type | Primary Antibody |
| Purity | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Conjugation | APC |
| Target | CD133 |
| Entrez | 8842 |
| UniProt ID | O43490 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-Prominin 1 antibody, anti-PROM1 antibody, ant Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Expiration Date | 12 months from date of receipt. |
Flow cytometry multicolor surface staining of human bone marrow cells stained using anti-human CD133 (W6B3C1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD34 (581) PE antibody (20 μl reagent per milion cells in 100 μl of cell suspension).
Separation of human CD133 positive CD34 positive stem cells (red-filled) from CD133 negative CD34 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human bone marrow cells stained using anti-human CD133 (W6B3C1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
Flow cytometry surface staining pattern of human bone marrow cells stained using anti-human CD133 CD133 (W6B3C1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
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