Anti-Hu CD123 Purified

• Catalog Number: orb154404
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD123 which is also known as IL-3 receptor subunit alpha, IL-3R-alpha and IL3RA, is a single-pass type I membrane protein which is a member of the type I cytokine receptor family and Type 5 subfamily. CD123 is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. CD123 is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes.
• Clonality: Monoclonal
• Clone Number: 6H6
• Tested applications: FC, IHC-Fr, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD123
• Entrez: 3563
• UniProt ID: P26951
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-IL3RA antibody, anti-MGC34174 antibody, anti-
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of CD123 positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD123 (6H6) purified antibody (concentration in sample 0.11 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD123 (6H6) purified antibody (concentration in sample 0.11 μg/ml, GAM APC).