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Anti-Hu CD123 PE
Catalog Number: orb154527
| Catalog Number | orb154527 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody conjugated to PE which recognizes CD123 that is also known as IL-3 receptor subunit alpha, IL-3R-alpha and IL3RA, is a single-pass type I membrane protein which is a member of the type I cytokine receptor family and Type 5 subfamily. CD123 is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. CD123 is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes. |
| Clonality | Monoclonal |
| Clone Number | 6H6 |
| Tested applications | FC |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Immunogen | IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells |
| Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Conjugation | PE |
| Target | CD123 |
| Entrez | 3563 |
| UniProt ID | P26951 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-IL3RA antibody, anti-MGC34174 antibody, anti- Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Expiration Date | 12 months from date of receipt. |
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD123 (6H6) PE antibody (10 µl reagent / 100 µl of peripheral whole blood) and anti-human CD45 (MEM-28) Pacific Blue™ antibody (10 µl reagent / 100 µl of peripheral whole blood).
Separation of human CD123 positive Basophil granulocytes(red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD123 (6H6) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD123 (6H6) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).
