Anti-HLA Class I Purified

• Catalog Number: orb44076
• Category: Antibodies
• Description: Mouse monoclonal antibody to HLA-A
• Clonality: Monoclonal
• Clone Number: MEM-147
• Tested applications: FC, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: PHA-activated peripheral blood lymphocytes
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1 μg/ml. Western blotting: Non-reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HLA Class I
• RRID: AB_10990068
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA A Antibody, anti-HLA B Antibody, anti-HLA
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1 μg/ml. Western blotting: Non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of human Ramos B cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb MEM-147 and goat anti-mouse IgG (H+L)-HRP conjugate.

Separation of human leukocytes stained using anti-HLA Class I (MEM-147) purified antibody (concentration in sample 1.67 µg/ml, GAM PE, red-filled) from human leukocytes unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (surface staining).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-HLA Class I (MEM-147) purified antibody (concentration in sample 1.67 µg/ml) GAM PE.