Anti-Her2 (trastuzumab biosimilar) mAb

• Catalog Number: orb1173652
• Category: Antibodies
• Description: Anti-Her2 (trastuzumab biosimilar)mAb
• Species/Host: Human Humanized
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG1
• Dilution range: ELISA 1:5000-10000|Flow Cyt 1:100
• Form/Appearance: Powder
• Conjugation: Unconjugated
• Target: Her2
• Hazard Information: Non-Toxic
• UniProt ID: P04626
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERBB2;CD340;HER-2/neu;HER2;MLN19;NEU;NGL;TKR1
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human Her2, His tagged protein (orb689435) can bind Anti-Her2 (trastuzumab biosimilar) mAb (orb1173652) in a linear range of 3.2-400 ng/ml.

ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human Her2 Protein, hFc Tag (orb1173800) can bind Anti-Her2 (trastuzumab biosimilar) mAb (orb1173652) in a linear range of 3.81-1730.10 ng/ml. In order to specifically detect orb1173652, mouse anti-human Fab-specific antibody was used as detection antibody.

Flow cytometry analysis with 15 μg/mL Anti-Her2 (trastuzumab biosimilar) mAb (orb1173652) on Expi293 cells transfected with Human Her2 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Flow cytometry analysis of antigen binding of anti-human Her2 mAb (orb1173652). (A) orb1173652 does not bind to Jurkat cells that do not express Her2. (B) A clear peak shift of orb1173652 was seen compared to the control when incubated with Her2-expressing MCF-7 cells, indicating strong binding of orb1173652 to Her2. Antibodies were incubated at 2 μg/mL.