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Anti-H-RAS Purified
Catalog Number: orb43873
| Catalog Number | orb43873 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to H-Ras. |
| Clonality | Monoclonal |
| Clone Number | H-RAS-03 |
| Tested applications | WB |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Immunogen | Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | H-RAS |
| Entrez | 3265 |
| UniProt ID | P01112 |
| RRID | AB_10993342 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-H-Ras antibody Read more... |
| Note | For research use only |
| Application notes | Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions. |
| Expiration Date | 12 months from date of receipt. |
Western blotting analysis of human H-Ras using mouse monoclonal antibody H-Ras-03 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-H-Ras monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for H-Ras at approximately 19 kDa.
