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Catalog Number | orb699855 |
---|---|
Category | Antibodies |
Description | Mouse monoclonalantibody against Granzyme B conjugated to FITC |
Clonality | Monoclonal |
Clone Number | CLB-GB11 |
Tested applications | FC |
Reactivity | Human, Primate |
Isotype | Mouse IgG1 |
Immunogen | Human NK cell line YT-INDY-derived granzyme B |
Antibody Type | Primary Antibody |
Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | FITC |
Target | Granzyme B |
Entrez | 3002 |
UniProt ID | P10144 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-GZMB antibody, anti-HLP antibody, anti-CTLA1 Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry multicolor intracellular staining of human lymphocytes stained using anti-human Granzyme B (CLB-GB11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
Separation of human CD3 negative Granzyme B positive lymphocytes (red-filled) from CD3 negative Granzyme B negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Granzyme B (CLB-GB11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Granzyme B (CLB-GB11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).