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Item 1 of 2
Anti-GAPDHS Purified
Catalog Number: orb43877
| Catalog Number | orb43877 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to Intra-Acrosomal Protein. |
| Clonality | Monoclonal |
| Clone Number | Hs-8 |
| Tested applications | FC, ICC, WB |
| Reactivity | Human, Porcine |
| Isotype | Mouse IgM |
| Immunogen | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| Conjugation | Unconjugated |
| Target | GAPDHS |
| Entrez | 26330 |
| UniProt ID | O14556 |
| RRID | AB_10995916 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Alternative names | Anti-Intra-Acrosomal Protein (Human antibody, anti Read more... |
| Note | For research use only |
| Application notes | Immunocytochemistry: Recommended dilution: 10 μg/ml; membrane permeabilization (acetone) is essential. Flow cytometry: Recommended dilution: 3-12 µg/ml. Intraacrosomal staining. |
| Expiration Date | 12 months from date of receipt. |
Separation of human GAPDHS positive cells (red-filled) from GAPDHS negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human sperm cells stained using anti-GAPDHS (Hs-8) purified antibody (concentration in sample 7.5 μg/ml) GAM APC.
Flow cytometry intracellular staining pattern of human sperm cells stained using anti-GAPDHS (Hs-8) purified antibody (concentration in sample 7.5 μg/ml) GAM APC.
