Anti-gamma-Tubulin Purified

• Catalog Number: orb44552
• Category: Antibodies
• Description: Mouse Monoclonal to gamma Tubulin.
• Clonality: Monoclonal
• Clone Number: TU-32
• Tested applications: ICC, WB
• Reactivity: Animal, Plant, Protozoa
• Isotype: Mouse IgG1
• Immunogen: human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: gamma-Tubulin
• Entrez: 7283
• UniProt ID: P23258
• RRID: AB_11012319
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-gamma-tubulin antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions. Immunocytochemistry: Methanol/acetone fixation required.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of differential reactivity of monoclonal antibodies to γ-tubulin with human γ-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human γ-tubulin 1 (γ-Tb1) or γ-tubulin 2 (γ-Tb2), probed with Abs to γ-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of γ-Tb1 or γ-Tb2 probed with Abs to γ-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.

Western blotting analysis of human gamma-tubulin using mouse monoclonal antibody TU-32 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-gamma-tubulin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for gamma-tubulin at approximately 46 kDa.