Anti-Galectin 3/LGALS3 Antibody

• Catalog Number: orb196251
• Category: Antibodies
• Description: Anti-Galectin 3/LGALS3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequences identity with mouse and rat Galectin 3, respectively.
• Antibody Type: Primary Antibody
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 26 kDa
• UniProt ID: P17931
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Galectin-3; Gal-3; 35 kDa lectin; Carbohydrate-bin
• Note: For research use only
• Application notes: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-Galectin-3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Galectin-3 using anti-Galectin-3 antibody.Lane 1:human HeLa cell; 2:human SiHa cell; 3:human U87 cell; 4:human Hacat cell; 5:human PC-3 cell; 6:human T47D cell.

IF analysis of Galectin-3 using anti-Galectin-3 antibody.Galectin-3 was detected in immunocytochemical section of U20S cell.

IF analysis of Galectin-3 using anti-Galectin-3 antibody. Galectin-3 was detected in an immunocytochemical section of SiHa cells.

IHC analysis of Galectin-3 using anti-Galectin-3 antibody.Galectin-3 was detected in paraffin-embedded section of Human Lung Cancer Tissue.

IHC analysis of Galectin-3 using anti-Galectin-3 antibody.Galectin-3 was detected in paraffin-embedded section of Human Placenta Tissue.