Anti-GAB1 (pY627) Antibody

• Catalog Number: orb1422802
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GAB1 (pY627).
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: GAB1
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
• Conjugation: Unconjugated
• UniProt ID: Q13480
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: GRB2-associated-binding protein 1; GRB2-associated
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of GAB1 (pY627) staining in HeLa Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of GAB1 (pY627) expression in Hela (A), U2OS (B), mouse lung (C), mouse testis (D), rat lung (E) whole cell lysates.