Anti-ERBB2 / HER2 / CD340 Reference Antibody (trastuzumab)

• Catalog Number: orb1806343
• Category: Antibodies
• Description: Anti-ERBB2 / HER2 / CD340 Reference Antibody is expressed from CHO. It has a predicted MW of 145.5 kDa.
• Clonality: Monoclonal
• Tested applications: ELISA, FA, FACS, Kinetics
• Reactivity: Human
• Isotype: IgG
• Purity: > 95%
• Conjugation: Unconjugated
• MW: 150 kDa
• Target: ERBB2 / HER2 / CD340
• Endotoxins: < 1 EU/mg
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100 mM Pro-Ac 20 mM Arg pH 5.0
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-ERBB2 / HER2 / CD340 Reference Antibody (trastuzumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-ERBB2 / HER2 / CD340 Reference Antibody (trastuzumab)is more than 95% ;determined by SEC-HPLC.

Immobilized human HER2; His at 2 μg/mL can bind Anti-ERBB2 / HER2 / CD340 Reference Antibody (trastuzumab);EC50=0.005735μg/mL.

BT474 cells were stained with Anti-ERBB2 / HER2 / CD340 Reference Antibody (trastuzumab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC175=0.3024 μg/mL.

Trastuzumab inhibited the tumor growth of BT474 on NCG mice. The result showed significant anti-tumor effects; with an tumor inhibition rate (TGI) of 88.5% at 10 mpk at D35.