Anti-erbB-2 (Her-2/neu) [4D5-8 (trastuzumab)]

• Catalog Number: orb1152528
• Category: Antibodies
• Description: Mouse monoclonal antibody to erbB-2
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: 4D5-8 (trastuzumab)
• Tested applications: ELISA, FC, IHC
• Reactivity: Human
• Isotype: Human IgG2a
• Immunogen: A431 cells (human epidermoid carcinoma) (over)expressing EGFR.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: erbB-2 (Her-2/neu)
• UniProt ID: P04626
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD340; Metastatic lymph node gene 19 protein (MLN
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of paraffin embedded human breast tissue using anti-erbB-2 antibody 4D5-8 (trastuzumab) Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab) (orb348902). Antigen retreival was achieved by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with orb348902 was carried out at 4 µg/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3, 3′-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 µg/ml.