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Anti-Eotaxin/Ccl11 Antibody
Catalog Number: orb1098112
| Catalog Number | orb1098112 |
|---|---|
| Category | Antibodies |
| Description | Anti-Eotaxin/Ccl11 Antibody. Tested in ELISA, IHC applications. This antibody reacts with Rat. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC |
| Reactivity | Rat |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived rat Eotaxin/Ccl11 recombinant protein (Position: H24-P97). |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 75 kDa |
| UniProt ID | P97545 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Bestrophin-1; TU15B; Vitelliform macular dystrophy Read more... |
| Note | For research use only |
| Application notes | Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Rat ELISA, 0.1-0.5 μg/ml, Rat. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
| Expiration Date | 12 months from date of receipt. |
IHC analysis of Eotaxin/Ccl11 using anti-Eotaxin/Ccl11 antibody. Eotaxin/Ccl11 was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Eotaxin/Ccl11 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
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