Anti-EGFR [Matuzumab]

• Catalog Number: orb1152514
• Category: Antibodies
• Description: Mouse monoclonal antibody to EGFR
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: Matuzumab
• Tested applications: Blocking, ELISA, FC, IF, WB
• Reactivity: Human
• Isotype: Human IgG1
• Immunogen: The parental mouse antibody was generated by immunizing BALB/c mice intraperitoneally with A431 cells in phosphate buffered saline (PBS). Later on the humanzied version of the antibody was created by grafting CDRs of the murine antibody onto human constant regions.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: EGFR
• UniProt ID: P00533
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: ErbB-1; ERBB1; HER1; EGF; Epidermal growth factor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Binding curve of anti-EGFR antibody Matuzumab (orb348869) to EGFR-Fc fusion protein ELISA Plate coated with human EGFR-Fc at a concentration of 5 µg/ml. A 3-fold serial dilution from 100 to 0.1 ng/ml was performed using orb348869. For detection, a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.

Flow-cytometry using the anti-EGFR antibody Matuzumab HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab (orb348922, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed A431 cells with anti-EGFR antibody Matuzumab. Immunofluorescence analysis of unpermeabilisd paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric rabbit version of Matuzumab (orb348922) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb348922, DAPI, merged channels and an isotype control. The isotype control was stained with unimmunised rabbit IgG followed by Alexa Fluor® 488 secondary antibody.