Anti-EGFR (Cetuximab biosimilar) mAb

• Catalog Number: orb757419
• Category: Antibodies
• Description: Anti-EGFR (Cetuximab biosimilar) mAb
• Species/Host: Chimeric
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG1
• Dilution range: ELISA 1:5000-10000
• Form/Appearance: Powder
• Conjugation: Unconjugated
• Target: EGFR
• UniProt ID: P00533
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EGFR;ERBB;ERBB1;HER1;PIG61;mENA
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human EGFR, His tagged protein orb689439 can bind Anti-EGFR Neutralizing antibody in a linear range of 0.64-80 ng/ml.

EGFR protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EGFR (Cetuximab) on Expi293 cells transfected with human EGFR (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram)

Flow cytometry analysis of antigen binding of anti-human EGFR mAb (orb757419). (A) A clear peak shift of orb757419 was seen compared to the control when incubated with EGFR-expressing Hela cells, indicating strong binding of orb757419 to EGFR. Antibodies were incubated at 2 μg/mL.

Flow cytometry analysis of antigen binding of anti-human EGFR mAb (orb757419). (A) orb757419 does not bind to Jurkat cells that do not express EGFR. (B) A clear peak shift of orb757419 was seen compared to the control when incubated with EGFR-expressing A431 cells, indicating strong binding of orb757419 to EGFR. Antibodies were incubated at 5 μg/mL.