Anti-dsRNA [1D3]

• Catalog Number: orb1089973
• Category: Antibodies
• Description: Mouse monoclonal antibody to dsRNA
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1D3
• Tested applications: ELISA, IHC, WB
• Reactivity: Virus
• Isotype: IgG1
• Immunogen: RDV-RNA-methylated bovine serum albumin complex.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: dsRNA
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: double-stranded RNA; double-stranded ribonucleic a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA using 1D3 and dsRNA. The plate was coated with dsRNA at 5 µg/ml. Serial dilutions were prepared over a range of 3000 ng/ml to 0.0169 ng/ml. For detection, HRP-labelled anti-mouse IgG antibody was used.

Immunofluorescence staining of fixed cells infected with Dengue 2 Virus with anti-dsRNA antibody 1D3. Immunofluorescence analysis of paraformaldehyde-fixed, triton-permeabilized cells infected with Dengue 2 virus stained with the chimeric rabbit IgG version of 1D3 (orb1089972) at 1/300 followed by Alexa 568 secondary antibody (red).

Immunofluorescence staining of Zika virus-infected A549 cells with anti-dsRNA antibody 1D3. Immunofluorescence analysis of Zika virus-infected A549 cells stained with the chimeric rabbit IgG version of 1D3 (orb1089972) followed by a secondary antibody. The nuclear stain is DAPI (blue). Panels show from left-right: orb1089972 (green) with DAPI and merged channels (orb1089972 (green), DAPI (blue), Zika's E protein (red)).