You have no items in your shopping cart.
Cart summary
Item 1 of 2
Item 1 of 2
Anti-Decorin/DCN Antibody
Catalog Number: orb221233
| Catalog Number | orb221233 |
|---|---|
| Category | Antibodies |
| Description | Anti-Decorin/DCN Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human Decorin recombinant protein (Position: D31-K359). Human Decorin shares 80% and 77% amino acid (aa) sequences identity with mouse and rat Decorin, respectively. |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 40-50 kDa, 80-140 kDa. |
| UniProt ID | P07585 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Decorin; Bone proteoglycan II; PG-S2; PG40; DCN; S Read more... |
| Note | For research use only |
| Application notes | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, HumanWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Expiration Date | 12 months from date of receipt. |
IHC analysis of Decorin using anti-Decorin antibody. Decorin was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Decorin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Western blot analysis of Decorin using anti-Decorin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Decorin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. Specific bands were detected for Decorin at approximately 40-50 KD and 80-140 KD. The expected band sizes for Decorin are at 40-50 KD and 80-140 KD.
- Item 1 of 2
