Anti-DDDDK-tag [M2.1]

• Catalog Number: orb396143
• Category: Antibodies
• Description: Mouse monoclonal antibody to DDDDK-tag.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: M2.1
• Tested applications: ELISA, IF, WB
• Isotype: Mouse IgG
• Immunogen: Interleukin 2 genetically fused to the peptided DYKDDDDK, corresponding to the DDDDK epitope tag.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: DDDDK-tag
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: FLAG-tag; Equivalent to FLAG antibodies from Sigma
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Brizzard, B. L. et al. Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution Biotechniques, 16, 730-735 (1994)

PubMed: 8024796

Binding of Anti-DDDDK antibody to Multitag protein Comparison of the binding of the anti-DDDDK antibody M2.1 (orb396135) and an equivalent antibody M2 from a competitor to Multitag protein. Wells were pre-coated with 5 ug/mg Multitag protein and then probed with serial dilutions of antibody followed by an HRP-conjugated goat anti-mouse antibody.

Binding curves of the rabbit IgG chimeric version of the Anti-DDDDK-tag antibody M2.1 (orb396143; blue line) and isotype control (orb256390, anti-fluorescein; orange line) to an ELISA plate coated with Anti-i-motif DNA [iMab] at a concentration of 2.5 ug/ml.